Table 2.

Multiphoton in vivo tools for microglia imaging.

Labeling approach	Options for multiphoton in vivo imaging
Histochemical labeling	Plant lectin conjugated with fluorophores (tomato lectin, isolectin IB4) (Brawek and Garaschuk, 2017)
Genetic labeling	Knock in CX3CR1-GFP mice (Jung et al., 2000) CX3CR1-EGFP mice (Nimmerjahn et al., 2005) CD11b-CreERT2;R26-tdTomato mice mice (Füger et al., 2017) CD11b-CreERT2;R26-tdTomato; Iba1-eGFP mice (Füger et al., 2017)
Viral labeling	Viral vectors: microRNA-9-regulated vector (Brawek and Garaschuk, 2017) Recombinant adeno-associated viruses (rAAV) (Rosario et al., 2016)
Calcium indicators	Oregon Green BAPTA-1 GCaMP2 (retroviral vector) (Seifert et al., 2011) Twitch-2B (lentiviral vector) (Brawek et al., 2017) GCaMP5G mice (genetically encoded) (Gee et al., 2014).