Figure 1.

Global expression patterns of SyGCaMP2-mCherry in SyG37 mouse brain. Arrays of epifluorescence images were taken from living, 400 μm thick coronal slices prepared from adult, wild-type (WT; left) and SyG37 mice (center and right) and stitched together as described in the methods section. The first two rows show images collected with excitation wavelengths of 470 (A,B) and 550 nm (C,D) using identical brightness settings for red and green images for quantitative comparison. Panels (E,G) show images of the same WT slices illustrated in panels (A,C) but with the brightness increased to reveal autofluorescence detail. Panels (F,H) shows the images in (B,D) merged and the SyGCaMP2 to mCherry fluorescence ratio respectively. A cold-hot look up table was applied to highlight differences in ratio. The right-hand panel of images show the cortical and hippocampal structures outlined with the dotted box in panel (A) magnified. The scale bars in panel (H) represent 2 mm (left) and 1 mm (right) respectively. Calibration bars for all images, with the exception of those in panels (E–G) which were scaled differently for illustrative purposes, are shown to the right-hand side.