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. 2018 Jul 19;12:209. doi: 10.3389/fncel.2018.00209

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Copyright © 2018 Al-Osta, Mucha, Pereda, Piqué-Gili, Okorocha, Thomas and Hartell.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of stimulus frequency and number on responses recorded from the s. radiatum of the CA1 region of SyG37 mice. Trains of 10 stimuli were applied at a fixed voltage at frequencies between 1 Hz and 100 Hz. In panel (A) responses over time are shown for SyGCaMP2 fluorescence extracted from ROIs placed over the s radiatum (sr). Darker gray levels are used to distinguish higher frequencies of stimulation. The means and SEM for the peak (B) and initial slopes (C) of responses are shown (n = 6 slices from four animals). Panels (D–F) illustrate the effects of changing the number of stimuli, delivered at a fixed intensity and frequency of 20 Hz. Darker gray levels are used to distinguish the effects of more stimuli (n = 7 slices from four animals).