Table 1.
Refinement of procedures in ex vivo platforms.
| Type of experiment | In vivo | Ex vivo |
|---|---|---|
| Cut (deafferentation/deefferentation) and reconstruction of neural connections (nerve regeneration) | Severe (invasive and painful surgery) | Non-recovery |
| Traumatic lesion | Compression/constriction injury | Non-recovery |
| Administration of drugs | Moderate (parenteral injection) Severe (intraventricular or intrathecal injection) |
Non-recovery |
| Studies on neurotoxicity | Severe | Non-recovery |
| Release of biologically active molecules | Severe (microinjection or microelectrode implant) | Non-recovery |
| Induction of inflammation | Moderate (intradermic injection) Severe (intrathecal injection) |
Non-recovery |
Severity is indicated according to current EU regulations (DIRECTIVE 2010/63/EU OF THE EUROPEAN PARLIAMENT AND OF THE COUNCIL of 22 September 2010 on the protection of animals used for scientific purposes).