TABLE 2 .
Strains and plasmids used in this study
| Strain or plasmid | Description | Reference |
|---|---|---|
| Strains | ||
| Wild-type EPEC | EPEC strain E2348/69, streptomycin resistant | 68 |
| EPEC ΔescR | Nonpolar deletion of escR | This study |
| EPEC ΔescS | Nonpolar deletion of escS | This study |
| EPEC ΔescT | Nonpolar deletion of escT | This study |
| EPEC ΔescN | Nonpolar deletion of escN | 38 |
| EPEC ΔescV | Nonpolar deletion of escV | 38 |
| EPEC ΔescU | Nonpolar deletion of escU | 21 |
| E. coli DH10B | For plasmid handling | 79 |
| E. coli FHK12 | E. coli strain in which ctx promoter was fused to lacZ gene | 72 |
| E. coli PD28 | malE-deficient E. coli strain | 77 |
| E. coli SM10λpir | Conjugating bacteria | 80 |
| EPEC escR-3HA | 3HA C-terminally tagged chromosomal escR | This study |
| Plasmids | ||
| pEscRWT-3HA (pACYC184) | 3HA C-terminally tagged EscR in pACYC184 | This study |
| pEscRWT-3HA (pSA10) | 3HA C-terminally tagged EscR in pSA10 | This study |
| pEscR-TM3ex-3HA (pSA10) | 3HA C-terminally tagged EscR with 7L9A sequence instead of original TMD3 in pSA10 | This study |
| pEscR-TM3exD171-3HA (pSA10) | 3HA C-terminally tagged EscR with 7L9A sequence instead of original TMD3 with aspartic acid in position 171 | This study |
| EscRY164A-3HA (pSA10) | 3HA C-terminally tagged EscR with Y164A mutation in pSA10 | This study |
| EscRD171A-3HA (pSA10) | 3HA C-terminally tagged EscR with D171A mutation in pSA10 | This study |
| EscRS175A-3HA (pSA10) | 3HA C-terminally tagged EscR with S175A mutation in pSA10 | This study |
| ToxR-GpA-MBP | GpA TMD sequence inserted between ToxR and MBP | 50 |
| ToxR-Tar-1-MBP | N-terminal TMD of E. coli Tar sequenc inserted between ToxR and MBP | 49 |
| ToxR-A16-MBP | Sequence of 16 alanine residues inserted between ToxR and MBP | 50 |
| ToxR-7L9A-MBP | Sequence of 7 leucine residues and 9 alanine residues inserted between ToxR and MBP | 51 |
| ToxR-TMD1-MBP | First TMD sequence of EscR inserted between ToxR and MBP | This study |
| ToxR-TMD2-MBP | Second TMD sequence of EscR inserted between ToxR and MBP | This study |
| ToxR-TMD3-MBP | Third TMD sequence of EscR inserted between ToxR and MBP | This study |
| ToxR-TMD3Y164A-MBP | Third TMD sequence of EscR with Y164A mutation inserted between ToxR and MBP | This study |
| ToxR-TMD3D171A-MBP | Third TMD sequence of EscR with D171A mutation inserted between ToxR and MBP | This study |
| ToxR-TMD3S175A-MBP | Third TMD sequence of EscR with S175A mutation inserted between ToxR and MBP | This study |
| pACYC184 | Cloning vector, Cmr Tcr | 81 |
| pSA10 | pKK177-3 derivative containing lacIq | 82 |
| pRE112 | Suicide vector for allelic exchange, Cmr | 83 |