TABLE 1.
Patterns of heterocysts produced by strains of Anabaenaa
| Strain (genotype) | Time following N− (h) | Mean % heterocysts ± SD | Mean no. of vegetative cells ± SEM | Avg % heterocyst occurrence ± SD (single, double, and multiple contiguous heterocysts) |
|---|---|---|---|---|
| Wild type | 24 | 9.1 ± 0.3 | 10.3 ± 0.5 | 96 ± 1.7, 4 ± 1.7 |
| 48 | 9.5 ± 0.3 | 11.5 ± 0.3 | 96.7 ± 1.5, 3.3 ± 1.5 | |
| 72 | 8.9 ± 0.2 | 12.7 ± 0.4 | 95.3 ± 0.6, 6.7 ± 0.6 | |
| UHM150 (ΔhetN) | 24 | 9.5 ± 0.5 | 10.4 ± 0.3 | 88.3 ± 3.8, 10.7 ± 2.1, 1 ± 1.7 |
| 48 | 16.2 ± 0.2 | 5.9 ± 0.2 | 41.7 ± 2.1, 33.3 ± 4.0, 25 ± 4.4 | |
| 72 | 16.1 ± 0.1 | 6.1 ± 0.5 | 29 ± 1, 29.11 ± 2.1, 42 ± 2.5 | |
| UHM114 (ΔpatS) | 24 | 21.3 ± 3.1 | 3.8 ± 0.5 | 51.6 ± 2.5, 40.8 ± 2.3, 7.7 ± 3.6 |
| 48 | 18.1 ± 2.4 | 4.5 ± 0.3 | 56.1 ± 7.6, 39.6 ± 8.1, 6.3 ± 3.6 | |
| 72 | 15.9 ± 1.8 | 5.9 ± 1.1 | 61.7 ± 0.7, 33.1 ± 1.8, 5.2 ± 2.1 | |
| UHM328 [ΔhetN(M1L)] | 24 | 9.7 ± 0.2 | 10.6 ± 1.2 | 93.3 ± 1.5, 6.7 ± 1.5 |
| 48 | 9.3 ± 0.2 | 11.6 ± 0.1 | 94.3 ± 2.1, 5.4 ± 1.5, 0.3 ± 0.6 | |
| 72 | 9.3 ± 0.4 | 11.3 ± 0.3 | 95 ± 1.7, 5 ± 1.7 | |
| UHM345 [ΔhetN(M119L)] | 24 | 10 ± 0.2 | 8.8 ± 0.4 | 82 ± 1, 14 ± 1, 4 ± 1 |
| 48 | 14.3 ± 0.3 | 7 ± 0.5 | 70.7 ± 3.1, 20.3 ± 2.3, 9 ± 3.5 | |
| 72 | 15.3 ± 0.6 | 6.4 ± 0.2 | 70 ± 1, 19.7 ± 3.5, 10.3 ± 3.1 | |
| UHM346 [ΔhetN(M129L/M130L)] | 24 | 10.2 ± 0.2 | 9.2 ± 0.3 | 95 ± 1.7, 5 ± 1.7 |
| 48 | 10 ± 0.6 | 9.7 ± 0.1 | 91 ± 0.6, 8.7 ± 0.6, 0.3 ± 1 | |
| 72 | 9.9 ± 0.5 | 10.5 ± 0.5 | 93 ± 1, 7 ± 1 | |
| UHM356 [ΔhetN(M1L,M119L)] | 24 | 9.2 ± 0.9 | 11.2 ± 0.7 | 93.3 ± 2.5, 6.7 ± 2.5 |
| 48 | 15.2 ± 1.1 | 6.2 ± 0.1 | 50.3 ± 3.8, 36.7 ± 3.2, 13 ± 2.7 | |
| 72 | 15 ± 0.6 | 6.4 ± 0.2 | 49.7 ± 3.5, 36.3 ± 4.6, 14 ± 2.7 | |
| UHM347 [ΔhetN(M1L,M129L/M130L)] | 24 | 10.3 ± 0.3 | 8.7 ± 0.2 | 95.3 ± 0.6, 4.7 ± 0.6 |
| 48 | 10.1 ± 0.3 | 9.4 ± 0.5 | 93 ± 1, 7 ± 1 | |
| 72 | 10.2 ± 0.4 | 10 ± 0.3 | 94 ± 1, 5.7 ± 1.2, 0.3 ± 0.6 | |
| UHM348 [ΔhetN(M119L,M129L/M130L)] | 24 | 10.6 ± 0.2 | 9.4 ± 0.5 | 86.7 ± 3.5, 12 ± 2.7, 1.3 ± 1.2 |
| 48 | 15.7 ± 1.0 | 6.4 ± 0.2 | 61.3 ± 3.2, 26.7 ± 0.6, 12 ± 3.6 | |
| 72 | 16.7 ± 1.1 | 6.5 ± 0.4 | 49 ± 7, 30 ± 2, 21 ± 5.2 | |
| UHM349 [ΔhetN(M1L,M119L,M129L/M130L)] | 24 | 10.9 ± 0.2 | 9.6 ± 0.1 | 82.3 ± 2.1, 14.7 ± 1.5, 3 ± 1 |
| 48 | 16.8 ± 0.7 | 5.9 ± 0.5 | 39 ± 3, 34.7 ± 6.1, 26.3 ± 4.7 | |
| 72 | 17.7 ± 0.5 | 5 ± 0.5 | 39.7 ± 2.1, 29.3 ± 3.8, 31 ± 4.6 | |
| UHM362 {ΔhetN(M1L,V64V[GTG to GTT],M129L/M130L)} | 24 | 9.3 ± 0.1 | 10.9 ± 0.3 | 97.7 ± 1.2, 2.3 ± 1.2 |
| 48 | 10.1 ± 0.3 | 9.1 ± 0.3 | 94.7 ± 2.1, 5.3 ± 2.1 | |
| 72 | 9.6 ± 0.2 | 9.2 ± 0.3 | 93 ± 1.7, 7 ± 1.7 | |
| UHM352 [ΔhetN(E131A)] | 24 | 9.3 ± 0.6 | 10.8 ± 0.5 | 96.3 ± 0.6, 3.7 ± 0.6 |
| 48 | 13.6 ± 0.6 | 7 ± 0.5 | 63.3 ± 9.3, 25 ± 7.9, 11.7 ± 1.5 | |
| 72 | 15.5 ± 1.2 | 6.6 ± 0.3 | 47.7 ± 1.2, 32 ± 3.6, 20.3 ± 4.5 | |
| UHM353 [ΔhetN(E131L)] | 24 | 10.7 ± 1.0 | 8.5 ± 0.8 | 91.7 ± 4.6, 7.7 ± 4.2, 0.7 ± 1.2 |
| 48 | 15 ± 0.6 | 6.5 ± 0.5 | 65.7 ± 4.6, 24.7 ± 7.6, 9.7 ± 3.1 | |
| 72 | 15.9 ± 0.8 | 5.8 ± 0.3 | 55.3 ± 2.5, 32 ± 1, 12.7 ± 2.1 | |
| UHM354 [ΔhetN(E131Q)] | 24 | 8.7 ± 0.7 | 11.8 ± 0.4 | 98 ± 1, 2 ± 1 |
| 48 | 10.3 ± 0.1 | 9.1 ± 0.3 | 97.7 ± 1.5, 2.3 ± 1.5 | |
| 72 | 10.7 ± 0.3 | 8.3 ± 0.2 | 93.7 ± 1.5, 6.3 ± 1.5 | |
| UHM357 (ΔhetN::patS) | 24 | 9.3 ± 0.8 | 10.6 ± 0.1 | 88.2 ± 3.5, 11.8 ± 3.5 |
| 48 | 11.4 ± 0.4 | 8.4 ± 0.3 | 56.3 ± 4.0, 17.4 ± 6.7, 7.3 ± 3.5 | |
| 72 | 11.13 ± 0.6 | 7.5 ± 0.9 | 52.3 ± 4.0, 18.3 ± 6.7, 10.4 ± 3.5 | |
| UHM358 (ΔpatS::hetN) | 24 | 0.3 ± 0.2 | ||
| 48 | 2.7 ± 0.1 | |||
| 72 | 3.2 ± 0.4 |
a
At the indicated times following nitrogen step-down, 500 cells were counted in triplicate, and total heterocysts are presented as means ± standard deviations. For heterocyst occurrences, the presence of single, double, or multiple contiguous heterocysts was determined for 300 heterocysts in triplicate and is presented as the average percentage ± standard deviation. The vegetative cells between heterocysts were counted for 300 intervals and are presented as the mean ± standard deviation of the mean.