TABLE 2.
Strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic(s)a | Source or reference |
|---|---|---|
| Anabaena strains | ||
| PCC 7120 | Wild type | Pasteur Culture Collection |
| UHM114 | ΔpatS | 32 |
| UHM150 | ΔhetN | 19 |
| UHM163 | ΔhetR(R250K) | 14 |
| UHM328 | ΔhetN(M1L) | This study |
| UHM345 | ΔhetN(M119L) | This study |
| UHM346 | ΔhetN(M129L/M130L) | This study |
| UHM347 | ΔhetN(M1L,M129L/M130L) | This study |
| UHM348 | ΔhetN(M119L,M129L/M130L) | This study |
| UHM349 | ΔhetN(M1L,M119L,M129L/M130L) | This study |
| UHM352 | ΔhetN(E131A) | This study |
| UHM353 | ΔhetN(E131L) | This study |
| UHM354 | ΔhetN(E131Q) | This study |
| UHM356 | ΔhetN(M1L,M119L) | This study |
| UHM357 | patS replacing hetN in the hetN locus | This study |
| UHM358 | hetN replacing patS in the patS locus | This study |
| UHM362 | ΔhetN(M1L,V64V[GTG to GTT],M129L/M130L) | This study |
| Plasmids | ||
| pAM504 | Shuttle vector for replication in E. coli and Anabaena; Kmr Nmr | 38 |
| pDR320 | pAM504 with PpetE-hetN | 12 |
| pDR382 | pRL277 to introduce hetN into the native locus | 19 |
| pPJAV153 | pAM504 with PpetE-YFP | 23 |
| pPJAV213 | pAM504 with PpetE | 27 |
| pRL277 | Suicide vector; Spr Smr | 39 |
| pAD120 | pAM504 with PhetN-hetN(M1L)-YFP | This study |
| pAD121 | pAM504 with PhetN-hetN(M119L)-YFP | This study |
| pAD122 | pAM504 with PhetN-hetN(M129L/M130L)-YFP | This study |
| pAD123 | pAM504 with PhetN-hetN(M1L,M119L)-YFP | This study |
| pAD124 | pAM504 with PhetN-hetN(M1L,M129L/M130L)-YFP | This study |
| pAD125 | pAM504 with PhetN-hetN(M119L,M129L/M130L)-YFP | This study |
| pAD126 | pAM504 with PhetN-hetN(M1L,M119L,M129L/M130L)-YFP | This study |
| pAD127 | pAM504 with PhetN-hetN-YFP | This study |
| pAD128 | pAM504 with PpetE-hetN(M1L)-YFP | This study |
| pAD129 | pAM504 with PpetE-hetN(M119L)-YFP | This study |
| pAD130 | pAM504 with PpetE-hetN(M129L/M130L)-YFP | This study |
| pAD131 | pAM504 with PpetE-hetN(M1L,M119L)-YFP | This study |
| pAD132 | pAM504 with PpetE-hetN(M1L,M129L/M130L)-YFP | This study |
| pAD133 | pAM504 with PpetE-hetN(M119L,M129L/M130L)-YFP | This study |
| pAD134 | pAM504 with PpetE-hetN(M1L,M119L,M129L/M130L)-YFP | This study |
| pAD135 | pAM504 with PpetE-hetN-YFP | This study |
| pAHB174 | pRL277 to make UHM362 | This study |
| pOR101 | pRL277 to make UHM328 | This study |
| pOR102 | pRL277 to make UHM345 | This study |
| pOR103 | pRL277 to make UHM346 | This study |
| pOR104 | pRL277 to make UHM356 | This study |
| pOR105 | pRL277 to make UHM347 | This study |
| pOR106 | pRL277 to make UHM348 | This study |
| pOR107 | pRL277 to make UHM349 | This study |
| pOR108 | pAM504 with PpetE-hetN(M1L) | This study |
| pOR109 | pAM504 with PpetE-hetN(M119L) | This study |
| pOR110 | pAM504 with PpetE-hetN(M129L/M130L) | This study |
| pOR111 | pAM504 with PpetE-hetN(M1L,M119L) | This study |
| pOR112 | pAM504 with PpetE-hetN(M1L,M129L/M130L) | This study |
| pOR113 | pAM504 with PpetE-hetN(M119L,M129L/M130L) | This study |
| pOR114 | pAM504 with PpetE-hetN(M1L,M119L,M129L/M130L) | This study |
| pOR115 | pRL277 to make UHM352 | This study |
| pOR116 | pRL277 to make UHM353 | This study |
| pPJAV348 | pRL277 to make UHM357 | This study |
| pPJAV349 | pRL277 to make UHM358 | This study |
| pPJAV369 | pRL277 to make UHM354 | This study |
a
Kmr, kanamycin resistance; Nmr, neomycin resistance; Spr, spectinomycin resistance; Smr, streptomycin resistance.