FIG 4.

Linear representations of the AlgO (A) and MucP (B) proteins and their mutant variants. Signal sequence (SS), zinc-binding (Zn-BS), protein-protein association (PDZ), transmembrane (TM), catalytic and tail-specific protease (TSP), and RIP motif domains are indicated. Changes in the sequence due to frameshifting or single-amino-acid changes are indicated in grey (AlgO) and pink (MucP), and the specific deletion in AlgO336 is indicated as a gap in the sequence. The positioning of MucP in the inner membrane is indicated at the bottom of panel B. The zinc-binding and RIP metalloprotease motifs are indicated as HEFGH and LDGG, respectively. The C1N and MRE β-loop domains (62) are situated between TM1 and TM2, respectively. The conserved motif of the MRE β-loop (63) is indicated as PLGG.