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. 2018 Jul 25;200(16):e00176-18. doi: 10.1128/JB.00176-18

FIG 4.

FIG 4

In-frame deletion of uhpC rescues 2DG-mediated inhibition of L. pneumophila replication in BMMs. (a) LP02 ΔflaA lux and LP02 ΔflaA ΔuhpC lux L. pneumophila replication in BMMs with or without 1.0 mM 2DG as measured by light emission (RLU). (b) LP02 ΔflaA and LP02 ΔflaA ΔuhpC L. pneumophila replication in BMMs with or without 1.0 mM 2DG as measured by CFU. (c) Replication of LP02 ΔflaA lux and LP02 ΔflaA ΔuhpC lux L. pneumophila complemented with pJB908 or pJB908 expressing wild-type (LP02) uhpC in BMMs with or without 0.5 mM 2DG as measured by light emission (RLU). (d) LP01 ΔflaA lux and LP01 ΔflaA ΔuhpC lux L. pneumophila replication in BMMs with or without 1.0 mM 2DG as measured by light emission (RLU). (e) LP01 ΔflaA lux and LP01 ΔflaA ΔuhpC lux L. pneumophila replication in Acanthamoeba castellanii as measured by light emission (RLU). ***, P < 0.001; ns, not significant (comparing growth curves by two-way ANOVA). Data are representative of at least two independent experiments. Symbols and bars represent means ± the standard deviations from at least three technical replicates.