Table 2. . Longitudinal and transverse 50 kHz electrical impedance myography and Cole parameters of the left gastrocnemius.

Direction of measurement	Impedance parameter	PBS (n = 8)	ActRIIB-mFc dose mg/kg			ANOVA p-values	Post-hoc p-value
			3.3 (n = 8)	6.6 (n = 8)	9.9 (n = 9)
Longitudinal	50 kHz phase	20.77 ± 0.525	21.04 ± 0.619	22.18 ± 0.601	22.63 ± 0.295	0.0451	ns ns ns
	50 kHz reactance	90.02 ± 3.49	80.66 ± 6.01	92.41 ± 4.72	89.00 ± 2.26	ns	ns ns ns
	50 kHz resistance	237.0 ± 4.98	207.7 ± 10.82	225.9 ± 7.29	213.4 ± 3.78	0.0317	0.0347 ns ns
Transverse	50 kHz phase	22.61 ± 0.567	25.13 ± 0.453	25.34 ± 0.499	25.97 ± 0.507	0.0004	0.0088 0.0043 0.0003
	50 kHz reactance	126.6 ± 6.50	135.5 ± 7.51	155.6 ± 12.35	134.5 ± 4.99	ns	ns ns ns
	50 kHz resistance	304.6 ± 15.14	288.5 ± 13.81	332.8 ± 34.94	275.5 ± 5.73	ns	ns ns ns
Longitudinal	fc (kHz)	37,705 ± 2475	31,873 ± 4760	30,127 ± 5150	31,482 ± 3219	ns	ns ns ns
	R0/Rinf	3.67 ± 0.206	3.68 ± 0.298	3.846 ± 0.229	4.34 ± 0.087	ns	ns ns ns
	α	0.7506 ± 0.0228	0.7093 ± 0.0116	0.7602 ± 0.0208	0.7075 ± 0.0082	ns	ns ns ns
Transverse	fc (kHz)	58,423 ± 4021	38,875 ± 3182	40,097 ± 2940	36,487 ± 2125	<0.0001	0.0007 0.0015 0.0001
	R0/Rinf	5.414 ± 0.344	6.065 ± 0.337	5.861 ± 0.346	6.385 ± 0.236	ns	ns ns ns
	α	0.7347 ± 0.0243	0.7131 ± 0.010	0.7481 ± 0.0254	0.7145 ± 0.0095	ns	ns ns ns

Phase shown in degrees, and reactance and resistance are shown in ohms. Data presented as mean ± standard error of the mean.

Post-hoc p-values are reported for the comparison between each individual ActRIIB-mFc dose versus the PBS control. None of the post-hoc p-values for the comparison between the various ActRIIB-mFc doses were significant.

ANOVA: analysis of variance; fc: Center frequency; ns: Not significant; PBS: Phosphate-buffered saline.