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. Author manuscript; available in PMC: 2018 Jul 26.
Published in final edited form as: Cell Rep. 2018 Jun 26;23(13):3759–3768. doi: 10.1016/j.celrep.2018.05.096

Figure 2. NMDAR Autoantibodies Induce a Time-Dependent Increase in the Receptor Content of the Surface NMDAR Nano-objects.

Figure 2

(A) Upper panels show a representative STORM image of the surface NMDAR (green) and PSD95 (magenta). Lower panels are higher magnifications of the white region, showing examples of synaptic (arrows) and extrasynaptic (arrowheads) NMDAR nano-objects.

(B) Schematic illustration representing the distinction used to identify synaptic versus extrasynaptic NMDAR nano-objects located within 200 nm of the center of the closest PSD95 nano-object versus farther away.

(C) Quantification of the number of localizations per NMDAR nano-object, a relative measure of the receptor content of the nano-objects, after 2, 6, or 24 hr of incubation with the control CSF (CSF−, dark gray), with patients’ CSF alone (CSF+, red), or in the presence of ephrin-B2 (Eph+CSF+, cyan). The box, line, and dot correspond to interquartile range (IQR, 25th–75th percentile), median, and mean, respectively (synaptic, n ≥ 835 nano-objects; extrasynaptic, n ≥ 2001 nano-objects; **p < 0.01, ***p < 0.001, ****p < 0.0001).

(D) Relative change in the number of localizations per nano-object obtained by normalizing the CSF+ means at each time to the corresponding CSF− means.

(See also Figure S3.