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. 2018 Jul 20;9:1610. doi: 10.3389/fmicb.2018.01610

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Copyright © 2018 Rühl, Haas, Seipel, Becker and Kletzin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Native AcPDO and effects of denaturants. (A) Non-denaturing 4–16% polyacrylamide gel with different amounts of AcPDO; M, Native marker liquid mix for BN/CN (Serva, Heidelberg, Germany); (B) Gel permeation chromatography of freshly prepared AcPDO (0.5 mg protein) with marker proteins; (C) Gel permeation chromatography of previously frozen AcPDO (0.1 mg protein) in the presence of different concentrations of guanidinium hydrochloride.