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Analysis of AcPDO using a MalPEG gel shift assay. (A) Coomassie-stained 10% Tris-tricine gel of the AcPDO wild type (10 μg/lane). (B) Western analysis using StrepMAP-Classic HRP-conjugated antibody. M, Marker in kiloDalton, NEM, sample derivatized with N-ethylmaleimide, DTT, sample reduced with dithiothreitol.
