. 2018 Jul 20;9:1610. doi: 10.3389/fmicb.2018.01610

Table 1.

Comparison of the activity measurements and kinetic constants obtained of PDOs from Ac. caldus C-SH12 and MTH-04.

Ac. caldus strain				C-SH12	MTH-04	MTH-04
Accession/locus tag				PRJEB24175	A5904_0790	A5904_0421
	Substrate	Co-substrate^a	Unit
Specific activity	1 mM GSH	2% S⁰	U/mg	61.6 ± 3.5	2.34^b	n.r.^c
	1 mM GSSH	–	U/mg	118 ± 10	n.r.	0.066
V_max	1 mM GSH	2% S⁰	U/mg	111.5	n.r.	n.r.
	1 mM GSSH	–	U/mg	122.3	n.r.	n.r.
K_M	GSH	2% S⁰	μM	≈500	n.r.	n.r.
	GSSH	–	μM	Not applicable^d	298 ± 13	267 ± 31
K_cat	GSH	2% S⁰	s⁻¹	181	n.r.	n.r.
	GSSH	–	s⁻¹	198	48.1	5.4
K_cat/K_M	GSH	2% S⁰	mM⁻¹ s⁻¹	361	n.r.	n.r.
	GSSH	–	–	Not applicable^d	161.4	20.2
Hill coefficient	GSSH	–	–	2.3 ± 0.22	n.r.	n.r.
Reference				This work	Wang et al., 2014;	Wu et al., 2017
					Wu et al., 2017

^aBoth added in the enzyme assay mixture. ^b0.8 mM GSH; 1 U of activity had been defined as 1 nmol sulfite formed per min, not 1 μmol (Wang et al., 2014). ^cn.r., not reported. ^dK_M was not calculated due to the sigmoidal curve (Figure 3).