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. 2018 Jul 19;9:1650. doi: 10.3389/fimmu.2018.01650

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Copyright © 2018 Vidyarthi, Khan, Agnihotri, Negi, Das, Aqdas, Chatterjee, Colegio, Tewari and Agrewala.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Signaling through TLR-3 increases macrophages capacity to activate CD4 T cells. M2a and M2c macrophages loaded with antigen were cocultured with efluor-labeled antigen-specific CD4 T cells isolated from lymph nodes of OVA immunized mice. The proliferation was assessed by eFluor-dye dilution assay. Number in the flow cytometry histogram indicates the CD4 gated efluor^lo cells. The results are also illustrated as bar graphs (side panel). The data expressed as mean ± SD are representative of two independent experiments (*p < 0.05).