Figure 7.

Images of cytospin slides of milk samples taken from glands inoculated with S. aureus. Slides were stained with May–Grünwald–Giemsa. Representative slides are shown. A Proliferation of staphylococci in the milk of goat #7 at 18 hpi, with bacterial clusters next to cells with altered nucleus. B At 18 hpi, the milk of goat #5 shows images of phagocytosis, much less bacteria, and many neutrophils with recognizable nucleus. C Milk sample of goat #4 at 23 hpi showing mainly intact neutrophils. The arrow indicates a neutrophil with ingested staphylococci. D Milk sample of goat #9 taken at 23 hpi, about 7 h before death; clusters of staphylococci are visible, and cells are damaged. E Milk samples of goat #2 at 36 hpi, showing intact neutrophils and mononuclear leukocytes and very few staphylococci (arrow). F Milk samples of goat #7 at 36 hpi, showing clusters of staphylococci and cells whose nucleus has the characteristic aspect of neutrophils intoxicated with the leukotoxin LukMF′ (arrow).