Table 1.
Bacterial strains, selected plasmids, and primers used in this study
| Strain, plasmid or primer target gene | Relevant characteristics | Reference or source |
|---|---|---|
| Escherichia coli strains | ||
| DH5α | supE44 ∆lacU169 (ϕ80 lacZ∆M15) recA hsdR17 recA1 endA1 gyrA96 thi-1 relA-1 | Invitrogen |
| SM10 | thi-1 thr leu tonA lacY supE recA:: RP4-2 tc:: Mu; lambda pir, KanR | Invitrogen |
| Pseudomonas chlororaphis strains | ||
| HT66 | Wild-type, SpR | This study |
| ΔpsrA | Mutant of HT66 in the psrA gene, KanR | This study |
| ΔrpeA | Mutant of HT66 in the rpeA gene, GmR | This study |
| Plasmids | ||
| pEX18Tc | Sucrose sensitive cloning vector, TcR | TAKARA |
| pEX-psrA | psrA from HT66 in pEX18Tc, TcR | This study |
| pEX-psrA-KanR | KanR cassette insertion from pBS(kan) in pEX-psrA; TcR,KanR | This study |
| pEX-rpeA-aacC1 | GmR cassette insertion from pUCGM in pEX-rpeA; TcR,GmR | Huang et al. [38] |
| Primer target gene | Primer sequences (5′–3′) | |
| psrA | psrA1: TTAAGCTTACGGTCGGGTCGTCGCTGCATA | HindIII |
| psrA2: AAGAGCTCCGCTGTTCATGCCACGGATAAAG | SacI | |
| rpeA | rpeAE1: TATTAATCTAGACCTGTTCAGCCGTTCCGAAT | XbaI |
| rpeAE2: AATTATGAATTC-CCACGCCCAGTTGATCCT | EcoRI | |
| KanR (kanamycin resistance gene) | pBS-Kan-R: TATAGCATGCTCAACCGTGGCTCCCTCA | SphI |
| pBS-Kan-F: TTAGCATGCCTTACTGTCATGCCATCCG | SphI | |
| aacC1 (gentamycin resistance gene) | Gm-R and Gm-F: AGAATCGATATCCCCGGGTACCGAG | ClaI |
aKanR, SpR, AmpR, GmR, and TcR represent kanamycin, spectinomycin, ampicillin, gentamycin and tetracycline resistance, respectively