Fig. 5. Effect of endosulfan on sperm DNA fragmentation. Mice sperm previously capacitated were preincubated with endosulfan AI or CFs and decondensed in the presence of H (2.3 μM) and GSH (10 mM) (A, B, C). Sperm DNA fragmentation was evaluated performing the TUNEL assay (D, E, F). A and D: mice sperm preincubated with endosulfan AI. B and E: sperm preincubated with endosulfan Master®. C and F: sperm preincubated with endosulfan Zebra Ciagro®. H: heparin, GSH: glutathione. The results are expressed as mean ± SEM (AI: n = 5, Master®: n = 4, Zebra Ciagro®: n = 5; *p < 0.05, ***p ≤ 0.0001).