Fig. 6. Correlation between in vitro sperm chromatin decondensation and DNA fragmentation. Mice sperm previously capacitated were preincubated with endosulfan or IVFM (control) and decondensed in the presence of GSH (10 mM) + H (2.3 μM). After that, the TUNEL assay was performed on each sample and the correlation was evaluated. A: Capacitated sperm incubated in the presence of IVFM and decondensed with GSH + H (r² = –0.4857, p = 0.3556). B: Capacitated sperm incubated in the presence of endosulfan AI and decondensed with GSH + H (r² = –0.5508, p = 0.2972). C: Capacitated sperm incubated in the presence of endosulfan Master® and decondensed with GSH + H (r² = 0.6571, p = 0.1750). D: Capacitated sperm incubated in the presence of endosulfan Zebra Ciagro® and decondensed with GSH + H (r² = –0.1160, p = 0.8028) (Spearman correlation, NS, n = 6).