Fig. 6. Effects of sodium hydrosulphide (NaHS, an H2S donor) on cell viability and LDH release in normal rat kidney proximal cells (NRK-52E) intoxicated by uranium. After the kidney cells were pretreated with different concentrations of NaHS (50, 100 or 200 μM) for 30 min and then intoxicated with uranyl acetate (400 μM) for 24 h, cell viability (A) was determined by MTT assay, and LDH leakage (B) was tested by a modification of a routine colorimetric laboratory method as described in the “Materials and methods” section. Values are expressed as the mean ± SE (n = 5). An asterisk represents a significant difference between cells treated with uranium alone and untreated cells; a sharp sign represents a significant difference between cells treated with uranium alone and uranium pulsing NaHS treated cells (Two-way ANOVA, *P < 0.05, **P < 0.01, #P < 0.05).
