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. 2018 Jun 5;25(1):1302–1318. doi: 10.1080/10717544.2018.1474974

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© 2018 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — In vitro cytotoxicity of Cur-VPDP, DOX-VPDP, or PDCP-NP against glioma spheroids-derived C6 cells at total drug concentrations of 0.4–6 μg/mL after 48 h (A) and 72 h (B), (C) CLSM images and flow cytometry (D) of glioma spheroids-derived C6 at 1 h, 4 h, and 8 h after treatment with the combinational Cur/DOX solution and PDCP-NP at Cur concentration of 1 μg/mL and DOX of 0.33 μg/mL, and the intracellular Cur and DOX concentrations of adherent (E) and spheroid-derived (F) cells were determined by HPLC after 8 h of incubation with various formulations. Data are shown as mean ± SD (n = 3), original magnification: 600×.