Fig. 5. H-PM-induced NF-κB activation depends on the MyD88 pathway. Saline, H-PM, or LPS (10 ng per mouse) was intratracheally administered to MyD88^–/– mice four times at 2-week intervals, and the mice were dissected 24 h after the last administration. Cell proliferation was measured as described in Fig. 1 (ConA stimulation, LPS stimulation for 72 h). The values shown are the mean relative cell proliferation as compared to that of PBS-stimulated cells of the control group. n.s., no statistically significant difference as compared to the control mice (n = 4 in each group). The left bar represents the data from saline-administered wild-type mice.