Fig. 2.

Relative abundance of the ether cross-linked peptide observed by mass spectrometry in a wt-R2lox aerobically reconstituted with only Fe or Mn and Fe and b V72I- and V72L-R2lox aerobically reconstituted with Mn and Fe. Samples were prepared in the dark. The area of the cross-linked peptide was normalized to the total protein area of the respective sample. Three (a) or two (b) replicate samples each were analyzed in technical triplicates, amounting to nine (a) and six (b) LCMS runs in total. Bars and error bars represent averages and standard deviations of the replicates