Figure 3.

3D TOF-SIMS tandem MS imaging of the human iPSC-derived neurons in the positive ion mode. Each 2D image in a row was extracted from the 3D image volume. The top row displays MS¹ images of the phosphocholine head group (C₅H₁₅NPO₄⁺), and the bottom row displays MS² TIC images of a molecule identified as the [M]⁺ of α-tocopherol, as a function of sputtered depth. The MS¹ and MS² data were collected simultaneously. The maximum counts per pixel are given in each panel. The soma and neurites of the cell are clearly observed in both the MS¹ and the MS² images