Table 1.
Molecular identification of honeybee pollen pellets collected in Hokkaido, northern Japan
| No. | Identified planta | Identification using each DNA barcoding region | |
|---|---|---|---|
| trnL-trnF | ITS2 | ||
| 1 | Actinidia polygama (Siebold et Zucc.) Planch. ex Maxim. | Actinidia polygama | Actinidia polygama |
| 2 | Angelica sp. | Angelica sp. | Angelica sp. |
| 3 | Artemisia montana (Nakai) Pamp. | Artemisia sp. | Artemisia montana |
| 4 | Asparagus officinalis L. | Asparagus officinalis | Asparagus officinalis |
| 5 | Chelidonium majus L. | Chelidonium majus | Chelidonium majus |
| 6 | Chenopodium album L. | Chenopodium album | Chenopodium sp. |
| 7 | Cirsium vulgare (Savi) Ten. | Cirsium vulgare | Cirsium vulgare |
| 8 | Commelina communis L.b | – | – |
| 9 | Cucurbita maxima Duchesne ex Lam. | – | Cucurbita maxima |
| 10 | Fagopyrum esculentum Moench | Fagopyrum esculentum | – |
| 11 | Fallopia sachalinensis (F. Schmidt) Ronse Decr. | Fallopia sachalinensis | – |
| 12 | Filipendula camtschatica (Pall.) Maxim. | Filipendula sp. | Filipendula camtschatica |
| 13 | Hydrangea paniculata Siebold | Hydrangea paniculata | Hydrangea paniculata |
| 14 | Hydrangea petiolaris Siebold et Zucc. | Hydrangea petiolaris | – |
| 15 | Hypochaeris radicata L. | Hypochaeris radicata | Hypochaeris radicata |
| 16 | Kalopanax septemlobus (Thunb.) Koidz. | Kalopanax septemlobus | Kalopanax septemlobus |
| 17 | Monochoria korsakowii Regel et Maackc | – | – |
| 18 | Oryza sativa L. | Oryza sativa | – |
| 19 | Parasenecio sp. | Parasenecio sp. | Senecioneae |
| 20 | Plantago lanceolata L. | Plantago sp. | Plantago lanceolata |
| 21 | Plantago sp. | – | Plantago sp. |
| 22 | Rudbeckia laciniata L.d | Rudbeckia hirta | Rudbeckia laciniata |
| 23 | Sagittaria trifolia L. | – | Sagittaria trifolia |
| 24 | Sinapis alba L. | – | Sinapis alba |
| 25 | Solanum nigrum L. | Solanum nigrum | Solanum nigrum |
| 26 | Solidago gigantea Aiton | Solidago gigantea | Solidago gigantea |
| 27 | Solidago virgaurea L. | Solidago sp. | Solidago virgaurea |
| 28 | Tilia sp. | Tilia sp. | Tilia sp. |
| 29 | Trifolium pratense L. | Trifolium sp. | Trifolium pratense |
| 30 | Trifolium repens L. | Trifolium repens | Trifolium repens |
| 31 | Zea mays L. | Zea mays | – |
aIn cases where identification using each DNA barcoding region terminated at different levels (nos. 3, 8, 12, 19, 20, 27, and 29), the more highly resolved identification was adopted
b,cIdentifiable using other DNA barcoding regions (bpsbA-trnH or cmatK). See Figs. S3, S4 in the ESM for the molecular phylogenetic trees
dBecause the trnL-trnF sequence of Rudbeckia laciniata was not registered in the database, identification using ITS2 was adopted. ITS2 molecular identification confirmed that Rudbeckia hirta was located in a different monophyletic group from that of pollen pellet no. 22 (Fig. S2 in the ESM)