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. 2018 Jan 5;20(8):1035–1045. doi: 10.1007/s12094-017-1821-0

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 2 — The expression of CXCL12 protein in colon cancer cell line after silencing of CXCL12 gene. Knockdown of CXCL12 by CXCL12 siRNA was confirmed by immunoblotting (a) in all four colon cancer cell lines. siRNA duplex oligoribonucleotides were transfected into cells for 48 h; the total RNA and proteins were extracted and then western blot. The grayscale values of the strips were measured by Image J software (b). Multiple comparisons were performed by one-way ANOVA followed by SNK test. Values are expressed as mean ± SD. Bars indicated SD