Fig. 4.

The effect of CXCL12 gene silencing on the invasive ability of colon cancer cells. A DLD-1 and HT-29 invasion by influence of CXCL12 siRNA was assessed by the BD Bio-Coat Matrigel invasion assay. The cells were incubated for 24 h; the invading cells were fixed and stained with Diff-Quick stain. The invading cells were counted in five random microscopic fields (×200). The invasive ability of DLD-1 transfected with CXCL12 siRNA group was significantly decreased compared with the untransfected and control siRNA groups (P < 0.01). The invasive ability of HT-29 that transfected CXCL12 siRNA group had no significant change compared with the untransfected and control siRNA groups. B The invasive figure is as follows: a DLD-1 cells untreated; b DLD-1 cells Control siRNA; c DLD-1 cells CXCL12 siRNA; d HT-29 cells untreated; e HT-29 cells Control siRNA; f HT-29 cells CXCL2 siRNA. Multiple comparisons used the method of one-way ANOVA and followed by the SNK test. Columns, relative invading number. Bars indicate SD, *P < 0.01