Fig. 5.

CXCL12 gene silencing influence on tube formation by HUVEC. Colon cancer cells were co-cultured with HUVEC and fibroblast using double-chamber method. All cells were cultured for total 11 days. The tubular formation was stained with anti-CD31 antibody by the protocols of manufacturer. The area of tubular formation was measured quantitatively over ten different fields for each condition using an image analyzer. A The image of angiogenesis shows a DLD-1 cells untreated; b DLD-1 cells Control siRNA; c DLD-1 cells CXCL2 siRNA; d HT-29 cells untreated; e HT-29 cells Control siRNA; f HT-29 cells CXCL12 siRNA. B The tubular formation of HUVEC was significantly inhibited by co-culture with CXCL12 siRNA DLD-1 cells compared with untransfected and control siRNA groups, respectively (P < 0.01). HT-29 of CXCL12 siRNA group had no significant change compared with the untransfected and control siRNA groups. Multiple comparisons used the method of one-way ANOVA and followed by the SNK test. Bars indicate SD, P < 0.01