Fig. 2.

In vivo effect of early (M1 to M5) or late (M5 to M9) vitamin D depletion/supplementation on neurogenesis in the dentate gyrus. Neurogenesis was assessed by quantifying the number of BrdU-positive (a) and doublecortin-positive cells (b) in male mice. A 4-month cholecalciferol supplementation, during the presymptomatic phase, improves cell proliferation (c) and neural progenitor cell proliferation and/or differentiation (d) in transgenic mice (n = 8–12). A 4-month cholecalciferol depletion, during the symptomatic phase, reduces neurogenesis in transgenic mice (e, f) (n = 6). *p < 0.05; **p < 0.01. Diets: 0VD = no vitamin D; NVD = normal dose of vitamin D; HVD = high dose of vitamin D