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. 2018 Jul 23;13:4333–4344. doi: 10.2147/IJN.S161908

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© 2018 Yang et al. This work is published and licensed by Dove Medical Press Limited

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(A) Proliferation inhibition assay of M-PLL/siRNA^Sur nanoparticles on 4T1 cells with or without NIR irradiation (n=6, mean±SD). (B) Representative cytotoxicity image of 4T1 cells incubated with PBS (a), naked siRNA^Sur (b), M-PLL/siRNA^N.C. (c), M-PLL/siRNA^Sur (d) or M-PLL/siRNA^Sur+NIR (e) by using calcein-AM (green, live cells) and propidium iodide (red, dead cells) staining method. Scale bar is 100 μm, magnification ×40. (C) Quantitatively apoptotic analysis of cells treated with different formulations by using Annexin V-FITC/PI staining assay. (D) Survivin protein levels in 4T1 cells after different treatment by ELISA method. Data were presented as nanogram of surviving protein per milligram of protein (n=3, mean±SD). **^,aP<0.01 by comparing M-PLL/siRNA^Sur (Laser off) with PBS and naked siRNA^Sur, ***^,bP<0.001 by comparing M-PLL/siRNA^Sur (Laser on) with PBS and naked siRNA^Sur and **^,cP<0.01 by comparing M-PLL/siRNA^Sur (Laser on) with M-PLL/siRNA^Sur (Laser off).

Abbreviations: M-PLL, melanin-poly-L-lysine; NIR, near infrared; siRNA, small interference RNA; PBS, phosphate-buffered solution; siRNA^N.C., siRNA of nonsense sequences; siRNA^Sur, survivin-targeted siRNA; ELISA, enzyme-linked immunosorbent assay; PI, propidium iodide.

(A) Proliferation inhibition assay of M-PLL/siRNA^Sur nanoparticles on 4T1 cells with or without NIR irradiation (n=6, mean±SD). (B) Representative cytotoxicity image of 4T1 cells incubated with PBS (a), naked siRNA^Sur (b), M-PLL/siRNA^N.C. (c), M-PLL/siRNA^Sur (d) or M-PLL/siRNA^Sur+NIR (e) by using calcein-AM (green, live cells) and propidium iodide (red, dead cells) staining method. Scale bar is 100 μm, magnification ×40. (C) Quantitatively apoptotic analysis of cells treated with different formulations by using Annexin V-FITC/PI staining assay. (D) Survivin protein levels in 4T1 cells after different treatment by ELISA method. Data were presented as nanogram of surviving protein per milligram of protein (n=3, mean±SD). **^,aP<0.01 by comparing M-PLL/siRNA^Sur (Laser off) with PBS and naked siRNA^Sur, ***^,bP<0.001 by comparing M-PLL/siRNA^Sur (Laser on) with PBS and naked siRNA^Sur and **^,cP<0.01 by comparing M-PLL/siRNA^Sur (Laser on) with M-PLL/siRNA^Sur (Laser off).

Abbreviations: M-PLL, melanin-poly-L-lysine; NIR, near infrared; siRNA, small interference RNA; PBS, phosphate-buffered solution; siRNA^N.C., siRNA of nonsense sequences; siRNA^Sur, survivin-targeted siRNA; ELISA, enzyme-linked immunosorbent assay; PI, propidium iodide.