Fig. 1.

Prep1 levels in adult C57BL/6J mouse brain regions. a Prep1 mRNA levels in the different brain regions of 6-month-old C57BL/6J mice. Bar represents the mean ± SEM of three independent experiments, in each of which reactions were performed in triplicate using the pooled total RNAs obtained from five mice. b Prep1 protein levels in brain regions from C57BL/6J mice. The autoradiographs are representative of four independent experiments. Asterisks denote statistically significant differences (*p < 0.05, **p < 0.01). c, d Immunofluorescence staining for Prep1 (green) and Calbindin (red) in the olfactory bulb at ×20 (c; scale bar, 20 μm) and ×63 (d; scale bar, 5 μm) magnification. The contour of a glomerulus (Gl) is indicated with a dashed line in (c). Periglomerular cells in (d) are shown with their Prep-1-ir nuclei (n) and Prep1/Calbindin-ir cytoplasm (c). CX, cortex; CB, cerebellum; c, cytoplasm; Gl, glomerulus; HIPP, hippocampus; HYP, hypothalamus; n, nucleus; OB, olfactory bulb