Table S1.
Primers used for screening for genes and PCR mapping
| PCR | Primer | Primer sequence (5′→3′) | Product length (kb) | Target | Reference |
|---|---|---|---|---|---|
| A | AF | GCACTTAGCCACCTATACGGCAG | 0.758 | blaCMY-2 for digoxigenin-labeled probes | 1 |
| AR | GCTTTTCAAGAATGCGCCAGG | ||||
| B | BF | AATGATGGCCAGGCTGTCTCC | – | 23SrRNA for digoxigenin-labeled probes | 2 |
| BR | CCGCCGTCGATATGAACTCTTG | ||||
| C | CF | GAAGGTTCTCAGAGCTGCAAC | 0.3 | RNAII for digoxigenin-labeled probes | This study |
| CR | GCCGCGTTTATCTCATTCCAC | ||||
| D | DF | ATCGTCTTTTACCGCCTGTCC | 3.1 | Screening for the plasmid of pSC137 | This study |
| DR | GTTAGCCCTATCCTGCATCGT | ||||
| E | EF | GTCATCGCTGGGAAATCGAAC | 3.8 | Junction between the 1S4 and yggR | 3 |
| ER | GCATAACGTCTCGGATCTACACC | ||||
| F | FF | AACTTGACGCCGAAGCCTA | 4.8 | Junction between the blaCMY-2 and orf9 | 3 |
| FR | TACGCCTGCAAAATATCACCA | ||||
| G | GF | TTTGTACTGCCAACGTATCCAA | 2.2 | Junction between the orf9 and ΔhsdR | 3 |
| GR | AAAGAACGGGAAATTGCCAAC | ||||
| H | HF | CCATAACAGCGGAATGACACC | 3.0 | Junction between RNAII and blaCMY-2 in the plasmid of pEC6413 | This study |
| HR | CAGAGCGCAGCATAACGAT | ||||
| I | IF | ACCGGGTTTTCATCCACGA | 1.12 | Junction between ΔhsdR and HP3 in the plasmid of pEC6413 | This study |
| IR | GTGCGTCTGACCAATATCCAC | ||||
| J | JF | CCATAACAGCGGAATGACACC | 3.2/4.8 | Junction between RNAII and ΔhsdR in the plasmids of pEC4103 or pEC5106 | This study |
| JR | AGGGCTTTACCTGTCAGCTC | ||||
| K | KF | CAGAGCGCAGCATAACGAT | 2.3/5.3 | Junction between blaCMY-2 and HP3 in the plasmid of pEC4103 or pEC5106 | This study |
| KR | CGTAATCCGTTGCCAGAGCC | ||||
| L | LF | TATTGTAGCATCGGTTTCCCA | 5.4 | Junction between the ISEcp1and ΔhsdR including pSC137 | This study |
| LR | GCGCGAACATACATATCCAGT | ||||
| M | MF | GCTGCTGACAGCCTCTTT | 0.197 | qCMY2 for quantitative real-time PCR assays | 4 |
| MR | GCGTGACTGGGTGGTTAT | ||||
| N | NF | GGCCGCAAGGTTAAAACTCAAATG | 0.243 | 16S rRNA for quantitative real-time PCR assays | 5 |
| NR | AACCGCTGGCAACAAAGGATAAGG |
Notes: F, forward primer; R, reverse primer. “L” represents reverse PCR primers and its 4 amplification region containing ISEcp1-pSC137-ΔhsdR.
Abbreviation: PCR, polymerase chain reaction.