Figure 2.

Quantification of germ cell types within the testes (ImageJ). H&E images of testicular tissue at ×21.6 magnification indicate that TCDD-lineage F₁ fish show a decreased area occupied by spermatozoa and an increased area of spermatogonia within seminiferous tubules. F₂ fish did not demonstrate differences in germ cell area due to ancestral TCDD exposure compared with controls. Top row consists of F₁ TCDD and control (DMSO) lineage testes. Second row consists of F₂ TCDD and control (DMSO) lineage testes. Scale bars in images are 100 µm length. Values in the table below indicate the mean area (µm²) ± SEM of each cell type within seminiferous tubules or the mean width of the germinal epithelium (µm) ± SEM for TCDD or control lineages. *Significant difference from control, as defined by p < .05. H&E, Hematoxylin and eosin; TCDD, 2,3,7,8-tetrachlorodibenzo-p-dioxin; DMSO, dimethyl sulfoxide; SEM, Standard Error of the Mean.