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. 2018 May 29;46(13):6627–6641. doi: 10.1093/nar/gky451

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© The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 4. — Analysis of the physical interaction between SisPINA-R206A/R147K/I199S and SisHjm by gel filtration. (A) Gel filtration profile of Hjm (red), SisPINA-R206A/R147K/I199S (black), and the mixture of Hjm and SisPINA-R206A/R147K/I199S (blue). (B) The SDS-PAGE of eluted fractions of SisPINA-R206A/R147KI/199S (top) and its mixture with Hjm (bottom). The procedure for the analysis was same as that for the analysis of the interaction between SisPINA and Hjm.