Figure 6.

Effect of SisPINA on the unwinding activity of SisHjm towards 3′-overhang (84 mer/34 mer) (A) and 5′-overhang (84 mer/34 mer) (B). The ‘*’ indicates labeling of the substrates at the 5′ end. Various concentrations of SisPINA (0, 10, 20, 30 and 40 nM, as hexamer) and 40 nM SisHjm (as monomer) were added into the reaction mixture and the reactions were carried out at 45°C for 25 min. The products were separated on 8% native PAGE gels and analyzed using a phosphor imager. (C) Quantification of the results in (A) and (B). The data were obtained with ImageJ software (NIH) and the values were calculated from at least three repeats.