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. 2018 May 29;46(13):6627–6641. doi: 10.1093/nar/gky451

Figure 8.

Figure 8.

SisPINA is able to unwind 3′-flap DNA. Lanes 2–4, replication fork without lagging strand; lanes 6–8, replication fork without leading strand; lanes 10–12, 3′-overhang DNA; lanes 14–16, 5′-overhang DNA. Lanes 1 and 5, 32P-labeled 36 nt ssDNA marker; Lanes 9 and 13, 32P-labeled 34 nt ssDNA markers. The ‘*’ indicates labeling of the substrates at the 5′ end. The concentrations of SisPINA (as hexamer) used for the analysis were indicated. All of the reactions were performed at 45°C for 30 min and the products were separated on 8% native page gels and analyzed with a phosphor imager.