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Holliday junction processing by SisPINA, SisHjm, and SisHjc. The cleavage of HSL (mobile) HJ substrate by SisHjc in the presence of SisPINA and SisHjm at indicated concentrations was analyzed by denatured (A) and native (B) gel electrophoresis. All of the reactions were carried out at 55°C for 30 min. The black arrow indicates band that is produced by HJ branch migration and cleavage. The ‘*’ indicates labeling of the substrates at the 5′ end.
