Table 2.
Enantiomer resolution and LODs (nM; ng/mL) obtained for 17 proteinogenic AAs using chiral CE-Flu
| Amino acida | Enantioresolution | LOD (nM)b | LOD (ng/mL)b |
|---|---|---|---|
| Alanine | 1.0 | 22 | 1.9 |
| Valine | 7.4 | 19 | 2.2 |
| Methionine | 3.9 | 39 | 5.8 |
| Threonine | 2.2 | 21 | 2.5 |
| Histidine | 3.4 | 98 | 15.2 |
| Isoleucine | 5.4 | 52 | 6.8 |
| Glutamic acid | 3.8 | 19 | 2.8 |
| Aspartic acid | 1.9 | 27 | 3.6 |
| Leucine | 5.4 | 37 | 4.8 |
| Phenylalanine | 8.8 | 28 | 4.6 |
| Tryptophan | 7.1 | 536 | 109.4 |
| Glycine | – | 27 | 2.0 |
| Proline | 1.5 | 38 | 4.3 |
| Serine | 2.1 | 16 | 1.7 |
| Asparagine | 2.1 | 15 | 1.9 |
| Glutamine | 1.7 | 14 | 2.0 |
| Arginine | 3.4 | 36 | 6.2 |
aInjected concentration, 500 nM per enantiomer (except tryptophan, 5000 nM)
bConcentration yielding a S/N of 3 as calculated for the d-enantiomer