Table 3.
Comparison with previously reported chiral CE-Flu methods for AAs
| Separation method and BGE | Derivatization agent and time | Excitation source and wavelength(s) | AAs with enantioresolution ≥ 1.0 | Lowest LOD (injected concentration/sample concentration; nM) | Application | Ref. |
|---|---|---|---|---|---|---|
| MEKC; 100 mM sodium tetraborate (pH 10.0), 80 mM SDS, 20 mM β-CD | FITC; overnight | Ar+ laser; 488 nm | Arg, Ala, Glu, Asp, Ser, Leu, Gln, Lys | 0.7/7 | CSF | [16] |
| MEKC; 100 mM sodium tetraborate (pH 10.0), 80 mM SDS. 20 mM β-CD | FITC; overnight | Ar+ laser; 488 nm | Arg, Ala, Glu, Asp, Ser | 160/3200 | Maize | [38] |
| MEKC; 100 mM sodium tetraborate (pH 9.4), 30 mM SDS. 20 mM β-CD | FITC; overnight | Ar+ laser; 488 nm | Arg, Asn, Ser, Ala, Glu, Asp | 0.3/1200 | Orange juice | [39] |
| MEKC; 100 mM sodium tetraborate (pH 9.7), 30 mM SDS, 20 mM β-CD | FITC; overnight | Ar+ laser; 488 nm | Arg, Ala, Glu, Asp | 16.6/8000 | Vinegars | [40] |
| MEKC; 150 mM Tris-borate (pH 9.0), 150 mM SDS with 60 mM HP-β-CD | NDA; 20 min | Violet LED; 395–425 nm | Asp | 0.25/2.5 | CSF, soymilk, beer | [41] |
| CE; 100 mM borate (pH 8.0), 8 mM DM-β-CD and 5 mM HPA-β-CD | NBD-F; 10 min | Ar+ laser; Ex, 488 nm | Glu, Asp | 50/600 | Brain | [42] |
| MEKC; 10 mM sodium borate (pH 9.1), 12 mM SC, 1.6% HSA, 10% methanol | DTAF; 30 min | Ar+ laser; Ex, 488 nm | Glu, Asp | 0.15/180 | Urine | [43] |
| MEKC; 80 mM sodium tetraborate (pH 9.2), 30 mM γ-CD, 30 mM STC, 5% acetonitrile | CFSE; 2 h | Ar+ laser; Ex, 488 nm | Ala, Glu, Asp, His, Ser, Leu, Val | 5/5 | Water from Mono Lake, CA | [44] |
| MEKC; 40 mM sodium tetraborate (pH 9.5), 30 mM SDS, 30 mM β-CD, 15% isopropanol | FMOC; 2 min | Xe-Hg lamp; 210–300 nm | Ala, Val, Met, Thr, His, Ile, Glu, Asp, Leu, Phe, Trp, Pro, Ser, Asn, Gln, Arg | 14/280 | CSF | this work |
HP-β-CD hydroxypropyl-β-cyclodextrin, DM-β-CD dimethyl-β-cyclodextrin, HPA-β-CD hydroxylpropylamino-β-cyclodextrin, SC sodium cholate, HSA human serum albumin, DTAF 5-(4,6-dichloro-s-triazin-2-ylamino) fluorescein, STC sodium taurocholate, CFSE 5-carboxyfluorescein succinimidyl ester, Ala alanine, Arg arginine, Asn asparagine, Asp aspartic acid, Glu glutamic acid, Gln glutamine, His histidine, Ile isoleucine, Leu leucine, Lys lysine, Met methionine, Phe phenylalanine, Pro proline, Ser serine, Thr threonine, Trp tryptophan, Val valine