Fig. 4. Effect of Red Wine Extract (RWE) on the Nrf2 nuclear translocation. Cells were incubated as described in Fig. 1. After 8 hours of incubation, nuclear protein extracts were obtained and then analysed by western blot using an anti-human Nrf2 antibody. In the bar graph, the Nrf2 content normalised to the lamin B1 level represents the mean ± SEM from at least six independent experiments and is expressed as a percentage of control cells (100%). Statistical significance: *p < 0.05, **p < 0.005 and ***p < 0.001 as compared to control cells; ^&p < 0.05, ^&&p < 0.005 and ^&&&p < 0.001 as compared to cells stimulated with cytokines.