Fig. 7. Effect of Red Wine Extract (RWE) on the GCLc gene expression. Cells were pre-treated with RWE (600 μg ml–1) for 30 min and then stimulated with the mix of cytokines. After 18 hours of incubation, GCLc mRNA production was evaluated by qRT-PCR, as described in the “Materials and methods” section, and expressed as fold increase above control cells. Statistical significance: ***p < 0.001, as compared to control cells; &&&p < 0.001 as compared to cells stimulated with cytokines.
