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Both monomeric and dimeric DapF can stimulate RppH activity. (A) Activity of RppH alone or in the presence of wild DapF, DapF (Y268A) mutant and BSA was detected by ion exchange chromatography. Activities of wild DapF and the DapF (Y268A) mutant are measured as control. (B) Activities of RppH or DapF variants were calculated and normalized to the activity of RppH alone and shown as columns. Each column represents two independent measurements, error bars represent standard deviations.
