Table 2.

Interaction of DapF and RppH measured by isothermal titration calorimetry

RppH (titrant)	DapF (titrate)	Stoichiometry (N)	K d (nM)	ΔH (kcal⋅mol-1)	ΔS (cal⋅mol-1⋅deg-1)
WT	WT	0.95	0.8 ± 0.4	−13.0 ± 0.1	−1.8
S128A	WT	0.85	12.8± 6.0	−12.5 ± 0.3	−6.0
R145A	WT	1.01	2.3 ± 1.0	−14.6 ± 0.1	−9.3
D142A	WT	1.05	4.8 ± 1.8	−11.0 ± 0.2	1.2
W130A	WT	—	—	—	—
WT	V19K	—	—	—	—
WT	T20A	0.97	4.2 ± 1.8	−14.4 ± 0.2	−9.8
WT	N22A	0.96	0.3 ± 0.1	−16.4 ± 0.1	−11.5
WT	F58R	—	—	—	—
WT	L89A	1.06	5.2 ± 0.9	−10.4 ± 0.1	3.0

— indicates that the interaction was not detectable.