Table 1.

Test Chemicals Used to Evaluate the Reliability of In Vitro Substrate Depletion Assays

Chemical	Structure	Log KOW	Previous Use in In Vitro Studies With RT-HEP and RT-S9
Cyclohexyl salicylate		4.70a	Laue et al. (2014)
Fenthion		4.09b	Fay et al. (2014b)
4-n-Nonylphenol		5.76b	Mingoia et al. (2010),Han et al. (2007, 2008, 2009) Fay et al. (2014b)
Deltamethrin		6.20b	Not tested
Methoxychlor		5.08b	Fay et al. (2014b)Bischof et al. (2016)
Pyrene		4.88b	Fay et al. (2014a, 2017) Mingoia et al. (2010),Nichols et al. (2013a,b, 2017)

^aMeasured value given by Laue et al. (2014).

^bMeasured values from the U.S. EPA EPI Suite experimental database (U.S. EPA, 2012).

Abbreviations: RT-HEP, cryopreserved rainbow trout hepatocytes; RT-S9, rainbow trout liver S9 fractions.