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. 2018 Jun 9;46(13):6773–6784. doi: 10.1093/nar/gky470

Figure 2.

Figure 2.

DNA supercoiling of a single topoisomer by gyrase containing two or one CTD(s). Supercoiling reactions were performed with 6 nM single topoisomer (start), 10 nM GyrA, and 20 nM GyrB in the presence of 1.5 mM ATP at 37°C, and reactions were stopped after 5 min. Gyrase with one and two CTDs decreases the linking number of the DNA in steps of two. ΔLk denotes the topoisomer ladder. Gyrase with one and two CTD(s) catalyzes negative DNA supercoiling by decreasing the linking number in steps of two.