Figure 5.

DNA cleavage by gyrase with two, one, or no CTD(s) in the presence of CFX. DNA cleavage reactions were performed at 37°C with 20 nM plasmid DNA, 200 nM GyrA, 800 nM GyrB, 1.5 mM ATP and increasing concentrations of CFX (0, 10, 100 or 250 μM) for 5 min. All enzymes show similar levels of DNA cleavage at all CFX concentrations tested, irrespective of the number of CTDs present.