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Relationship between abundance and the AK codon at the beginning of each permuted genes. A comparison of the number of (A) P and (B) AP sequences before (top) and after selecting (bottom) for biological activity. The residue position represents the AK residue found at the beginning of each ORF regardless of orientation. Only those ORFs encoding in frame variants are shown. In cases where a P or AP variant was absent, black bars are shown below the x-axis.
