Figure 6.
Enrichment of parallel sequences following selection. The log2(fold change) in sequence abundances of the AP (open symbols) and P variants (closed symbols). The significance of P variant enrichment obtained using the negative binomial model (PVNB) is colored as a function of the P-value obtained with the variants presenting values >0.01 in red, variants having values ≤10−300 in black and those variants displaying intermediate values shaded as indicated in the bar. The black line represents the mean dilution for AP variants relative to their initial abundance in the unselected library, while the dashed line represents two standard deviations greater than the mean. Variants not observed in the selected library (infinitely diluted) are plotted in the shaded region.