Table 1.
Abundance of sequence reads within each library
| Unselected | Selected | S/US ratio | |
|---|---|---|---|
| Total reads | 3 477 712 | 3 934 541 | 1.13 |
| +9 bp repeat | 402 805 | 528 526 | 1.31 |
| +start/stop motif | 298 707 | 405 857 | 1.36 |
| +start motif | 171 922 | 216 079 | 1.26 |
| +stop motif | 126 785 | 189 778 | 1.50 |
| +11 bp AK gene | 236 324 | 327 908 | 1.42 |
| all in frame AK gene | 114 754 | 327 908 | 2.86 |
| in frame P variants | 59 494 | 326 503 | 5.49 |
| in frame AP variants | 55 260 | 1405 | 0.03 |
| all +1 frame AK gene | 82 085 | 4373 | 0.05 |
| +1 frame P variants | 42 719 | 3581 | 0.08 |
| +1 frame AP variants | 39 366 | 792 | 0.02 |
| all −1 frame AK gene | 39 485 | 2727 | 0.07 |
| −1 frame P variants | 21 909 | 2398 | 0.11 |
| −1 frame AP variants | 17 576 | 329 | 0.02 |
The data generated by MiSeq analysis, including the total sequence reads having the bar code corresponding to the unselected and selected libraries, the number of reads that contain a 9 bp motif in the permuteposon (GCCGCTCAA and TTGAGCGGC), either the 54 bp start or stop motifs shown in Figure 2, and a start or stop motif and at least 11 bp of adjacent AK gene sequence. Among the variants containing a start (or stop) motif and 11 bp of adjacent AK sequence, we list the number of P and AP that are in frame, −1 frame, and +1 frame.