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. 2018 May 4;46(13):6841–6856. doi: 10.1093/nar/gky327

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© The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 8. — DapF stimulation of RppH reactivity with triphosphorylated RNA substrates. (A) Variable effect of DapF on RppH reactivity with triphosphorylated RNA substrates of different lengths. Pyrophosphate release was quantified by a colorimetric assay. RppH alone, black lines and circles; RppH complexed with DapF, red lines and circles. The RNA substrates were: pppAGU (3 nt), pppAGUUUU (6 nt), pppAGUUUUU (7 nt), and pppAGUUUUUUG (9 nt). Error bars represent standard deviations. Insets: the same data plotted semilogarithmically. (B) Comparison of the reaction time courses for RppH in the presence of DapF, as shown in (A). 3-, 6-, 7- and 9-nt RNAs are shown in black, red, blue and purple colors, respectively. (C) Rate constants of the reactions in (A). SD, standard deviations, n = 2.